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WormBase Tree Display for Expr_pattern: Expr2557

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Name Class

Expr2557Expression_ofGeneWBGene00000839
Reflects_endogenous_expression_ofWBGene00000839
Expression_dataLife_stageWBls:0000024
WBls:0000003
WBls:0000038
WBls:0000027
WBls:0000035
WBls:0000041
Anatomy_termWBbt:0005772Certain
WBbt:0005784Certain
TypeIn_situAntisense and sense digoxigenin-labelled RNA probes were created from full-length cul-4 cDNA using Ambion MegaScript T7 and T3 kits with digoxigenin-11-UTP. In situ hybridization was performed on whole animals, embryos or gonad arms dissected from hermaphrodites 2 days post-injection.
PatternIn C. elegans, the cul-4 gene is expressed throughout development. In adult hermaphrodites, cul-4 messenger RNA is observed primarily in the germ line, with transient expression in the intestine of young adults. cul-4 maternal mRNA is present in early embryos and decreases during embryogenesis. In larvae, cul-4 is broadly expressed, with high levels in proliferating tissues, notably in the intestine and germ line.
ReferenceWBPaper00005912