WormBase Tree Display for Expr_pattern: Expr2688
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| Expr2688 | Expression_of | Gene | WBGene00006810 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006810 | |||
| Expression_data | Life_stage | WBls:0000018 | ||
| WBls:0000019 | ||||
| WBls:0000020 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005319 | Certain | |||
| WBbt:0005813 | Certain | |||
| GO_term | GO:0042643 | |||
| Subcellular_localization | In 1.5-fold embryos: The UNC-78-positive regions were adjacent to the nascent myofibrils in body wall muscle where the muscle-specific myosin heavy chain myoA was localized, suggesting that UNC-78 is localized in the diffuse cytoplasm of the body wall muscle cells. | |||
| In later embryos, in the pharynx and spermatheca, subcellular localization of UNC-78 was not clear owing to poor penetration of the antibody into these tissues. In the body wall muscle, UNC-78 was localized in a striated pattern that was co-localized with actin but not with the myosin heavy chain myoA. However, since the striation of UNC-78 was not as sharp as that of actin, it was difficult to determine precise location of UNC-78 within the thin filaments. | ||||
| Type | Antibody | Polyclonal rabbit peptide antibody. A synthetic peptide CAGGSGVDSSKAVAN corresponding to residues 395-408 of UNC-78 plus additional cysteine at the N terminus was synthesized to raise rabbit antisera. | ||
| Pattern | Immunolocalization of UNC-78 revealed that UNC-78 is expressed in a tissue-specific manner. In embryos, faint staining of UNC-78 was first detected in a subset of cells at the 1.5-fold stage (~350 minutes after the first cell division). In the later embryonic stages, UNC-78 was expressed in the body wall muscle and also more strongly in the pharynx. In adults, UNC-78 was expressed in the body wall muscle, pharynx and spermatheca. | |||
| Picture | WBPicture0000009051 | |||
| WBPicture0000009052 | ||||
| Remark | Staining of unc-78 null mutants with anti-UNC-78 antibody did not yield these patterns, indicating that striated staining is specific for reactivity with UNC-78. | |||
| Reference | WBPaper00006108 | |||
| Antibody_info | WBAntibody00000674 |
