WormBase Tree Display for Expr_pattern: Expr2707
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Expr2707 | Expression_of | Gene | WBGene00004777 | ||
---|---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00004777 | ||||
Homol | Homol_homol | C02D4:Expr | |||
Expression_data | Anatomy_term | WBbt:0003724 | Certain | ||
WBbt:0003744 | Certain | ||||
WBbt:0003811 | Certain | ||||
WBbt:0003812 | Certain | ||||
WBbt:0003848 | Certain | ||||
WBbt:0003849 | Certain | ||||
WBbt:0003871 | Certain | ||||
WBbt:0003872 | Certain | ||||
WBbt:0003937 | Certain | ||||
WBbt:0003938 | Certain | ||||
WBbt:0003939 | Certain | ||||
WBbt:0003940 | Certain | ||||
WBbt:0003941 | Certain | ||||
WBbt:0003957 | Certain | ||||
WBbt:0003958 | Certain | ||||
WBbt:0003959 | Certain | ||||
WBbt:0003960 | Certain | ||||
WBbt:0003961 | Certain | ||||
WBbt:0003963 | Certain | ||||
WBbt:0004070 | Certain | ||||
WBbt:0004088 | Certain | ||||
WBbt:0004090 | Certain | ||||
WBbt:0004092 | Certain | ||||
WBbt:0004094 | Certain | ||||
WBbt:0004444 | Certain | ||||
WBbt:0004445 | Certain | ||||
WBbt:0004446 | Certain | ||||
WBbt:0004457 | Certain | ||||
WBbt:0004491 | Certain | ||||
WBbt:0004493 | Certain | ||||
WBbt:0004823 | Certain | ||||
WBbt:0004857 | Certain | ||||
WBbt:0004947 | Certain | ||||
WBbt:0004949 | Certain | ||||
WBbt:0004983 | Certain | ||||
WBbt:0004985 | Certain | ||||
WBbt:0004987 | Certain | ||||
WBbt:0004989 | Certain | ||||
WBbt:0004991 | Certain | ||||
WBbt:0004993 | Certain | ||||
WBbt:0004997 | Certain | ||||
WBbt:0004998 | Certain | ||||
WBbt:0004999 | Certain | ||||
WBbt:0005023 | Certain | ||||
WBbt:0005025 | Certain | ||||
WBbt:0005310 | Uncertain | ||||
Remark | Expression observed in several posterior neurons, likely to be CP neurons. | ||||
WBbt:0005776 | Certain | ||||
WBbt:0005813 | Partial | ||||
Remark | ventral body wall muscles | ||||
WBbt:0005815 | Certain | ||||
Type | Reporter_gene | [ser-2::gfp] transcriptional fusion constructs. Ser-2 reporter constructs were generated by using a PCR fusion protocol, using pPD95.75 as a template for green fluorescent protein (gfp). For all gfp fusion primers listed, gfp vector sequence is indicated in lowercase, and gene-specific sequence is indicated in uppercase. | |||
[ser-2::gfp] translational fusion. A translational fusion of the whole ser-2 locus to gfp was created by using an in vivo recombination technique. Specifically, two overlapping PCR fragments, one containing the 5' part of a locus, the other containing the remainder of the locus PCR-fused to gfp, were coinjected into the worm. Recombination of these two fragments via the homologous region leads to the expression of a full-length ser-2::gfp fusion. | |||||
Pattern | Expression using the upstream regulatory regions of exon 1bc (ser-2prom2::gfp) is mostly restricted to the AIYL/R, AIZL/R, RID, DVA, BDUL/R, SIADL/R, and SIAVL/R interneurons. Less consistent expression is observed in PVT. In addition, expression is observed in the RMEL/R motor neurons. Outside the nervous system, expression can be observed in the excretory gland cells. | ||||
No more transcriptional regulatory information is contained within intronic regions by generating a fusion of gfp to the full coding genomic ser-2 locus using an in vivo recombination technique([ser-2::gfp] translational fusion. Transgenic animals expressing such a construct show an expression pattern similar to the one observed with the ser-2prom1::gfp construct. | |||||
The upstream regulatory region of the third splice form, containing exon 1d(ser-2prom3::gfp), drives expression exclusively in two sensory neuron classes, OLL(L/R) and PVD(L/R). | |||||
ser-2prom1::gfp is expressed in the AIY interneuron class and a set of unidentified neurons. These neurons were identified as head and tail interneuron classes, namely AVHL/R, AUAL/R, AIYL/R, RICL/R, SABVL/R, RID, RIAL/R, SABD, SDQ, CANL/R, DA9, LUAL/R, ALNL/R, and PVCL/R. In addition to its expression in neurons, ser-2prom1::gfp is also expressed in pharyngeal cells (NSM neurons and pm1/6 muscles) and in head muscles. In males, expression can be observed in posterior dorsal and ventral body wall muscles, the male-specific diagonal muscles, and several posterior neurons likely to be CP neurons. | |||||
Reference | WBPaper00006180 | ||||
Transgene | WBTransgene00001598 | ||||
WBTransgene00001612 | |||||
WBTransgene00007053 |