WormBase Tree Display for Expr_pattern: Expr2922
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| Expr2922 | Expression_of | Gene | WBGene00004953 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004953 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| Anatomy_term | WBbt:0005175 | ||
| GO_term | GO:0005814 | ||
| GO:0000242 | |||
| Subcellular_localization | SPD-2 not only associates with centrosomes, but it does so at nearly all of the developmental stages examined. In the hermaphrodite gonad, SPD-2 localizes as discrete perinuclear foci in the mitotic portion of the germline. These foci were also evident during the early stages of oogenesis but were absent in mature oocytes. In contrast, mature sperm retained these SPD-2 foci with each male gamete containing a single dot adjacent to the nucleus. Similarly, in meiotic stage embryos, a single SPD-2-positive dot was observed next to the male pronucleus. In slightly older embryos, one or two perinuclear foci of SPD-2 staining were observed. The intensity of the foci increased with the age of the embryos and at mitosis both spindle poles were brightly stained. As embryos progressed through anaphase and telophase, the intensity of staining gradually diminished. Careful examination of the staining pattern at this stage revealed a diffuse area of SPD-2 material centered on one or two very bright dots. To determine if these central dots corresponded to the position of centrioles, SPD-2 distribution was examined in a strain expressing a GFP-tagged version of the centriole marker SAS-4 and it was found that the SPD-2 and SAS-4-positive dots coincided. SPD-2 probably localizes to both centrioles as well the pericentriolar region. Centrosome staining was also evident in older embryos and SPD-2-positive puncta were seen in young larvae, suggesting that SPD-2 is present at the centrosome throughout the entire life cycle. SPD-2 staining, however, was not restricted to the centrosome. Varying amounts of SPD-2 were also noticed present in the cytoplasm and nuclei of oocytes and embryos. | ||
| Type | Antibody | ||
| Picture | WBPicture0000009934 | ||
| Reference | WBPaper00013411 | ||
| Antibody_info | WBAntibody00000889 |
