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WormBase Tree Display for Expr_pattern: Expr2967

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Name Class

Expr2967Expression_ofGeneWBGene00001709
Reflects_endogenous_expression_ofWBGene00001709
Expression_dataLife_stageWBls:0000024
WBls:0000003
WBls:0000038
WBls:0000027
WBls:0000035
WBls:0000041
Anatomy_termWBbt:0003889Certain
WBbt:0003890Certain
WBbt:0005113Partial
WBbt:0005300Certain
WBbt:0005759Partial
WBbt:0005821Certain
TypeAntibodyA monoclonal antibody raised against mammalian GRK2/3 was used to confirm the putative Ce-GRK-2 cellular expression pattern. Although endogenous Ce-GRK-2 was below the level of detection using this antibody, Ce-GRK-2 was detected in transgenic animals overexpressing Ce-GRK-2 from the genomic rescue fragment.
Reporter_gene
PatternCe-grk-2::gfp reporter expression was observed in embryos as early as the 20 to 30 cell stage and persisted throughout development and into adulthood. The Ce-grk-2::gfp reporter was expressed in many neurons of adult animals, including the ASH neurons and other sensory neurons, many interneurons, and motor neurons of the ventral nerve cord. Expression was also observed in vulval muscles.
The GFP reporter and antisera expression patterns were nearly identical, but no immunoreactivity was observed in the vulval muscles.
PictureWBPicture0000009639
ReferenceWBPaper00013467
TransgeneWBTransgene00028278
Antibody_infoWBAntibody00000898