WormBase Tree Display for Expr_pattern: Expr3187
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| Expr3187 | Expression_of | Gene | WBGene00004063 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004063 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000020 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0005319 | Certain | ||
| WBbt:0005753 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0006794 | Certain | |||
| GO_term | GO:0005794 | |||
| GO:0005829 | ||||
| Subcellular_localization | The GFP was found to be highly diffused in the worms harboring pPMR pro::gfp, but in worms expressing pPMR pro & cDNA::gfp, GFP appeared to be punctated and was confined to cytosolic region. This difference is likely to reflect the differences in the sub-cellular localization. pPMR pro::manII DsRed was injected into the transgenic worm already expressing CePMR-1::GFP fusion protein. The pPMR pro::manII DsRed, which encodes a part of alpha mannosidase II (a Golgi resident protein), was expressed under the control of pmr-1 promoter. The localization of CePMR-1 is completely superimposable with that of mannosidase, confirming the localization of CePMR-1 at Golgi apparatus. Similar pattern was observed in intestine and spermatheca. | |||
| Type | Reporter_gene | |||
| Pattern | Transgenic worms containing the pmr-1::gfp construct showed GFP expression beginning as early as 3-fold stage and continued to be expressed throughout larval and adult stages. Expression was spatially confined to hypodermal seam cells, spermatheca, and intestine. When pPMR pro & cDNA::gfp was injected, transgenic worm showed essentially the same expression pattern, but different subcellular localization. Taken together, CePMR-1 is expressed in hypodermal seam cells, intestinal cells and spermatheca and male gonad; subcellularly it is localized at Golgi complex. | |||
| Reference | WBPaper00024915 | |||
| Transgene | WBTransgene00028427 |
