WormBase Tree Display for Expr_pattern: Expr3269
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| Expr3269 | Expression_of | Gene | WBGene00004859 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004859 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005733 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005812 | Certain | |||
| Type | Reporter_gene | |||
| Pattern | A second GFP fusion construct pPDW06-9, which contains the same promoter for W06B3.2c and the entire genomic region of W06B3.2a/c except for the termination codon, rescued the phenotypes of the sma-5 mutant, and looks to be expressed in the same regions as was pPDW06-1a. Fusion protein is localized throughout the intestine. | |||
| The third construct pPDW06-c carries the 4.9 kb sequence upstream of exon 1 and a GFP gene. GFP in this promoter fusion is expressed in hypodermis and pharynx. | ||||
| pPDW06-1a expresses a GFP fusion with N-terminal five amino acids of W06B3.2a/c under a control of promoter for W06B3.2c of 1.6 kb. GFP expression was found in intestine and excretory cell, in all stages of a transgenic line carrying extrachromosomal arrays of pPDW06-1a. In the intestine, the four most anterior cells show stronger GFP expression, although entire intestine is fluorescent. The expression in the excretory cell was confirmed by comparison with the expression pattern of vha-1p::gfp that is predominantly expressed in the excretory cell. GFP in the excretory cell is seen continuously along the lateral lines for most of the length of the worm. Weak expression in hypodermis is also seen. | ||||
| Picture | WBPicture0000007639 | |||
| Reference | WBPaper00025246 | |||
| Transgene | WBTransgene00028491 |
