WormBase Tree Display for Expr_pattern: Expr3398
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| Expr3398 | Expression_of | Gene | WBGene00002055 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002055 | ||||
| Expression_data | Life_stage (11) | ||||
| Anatomy_term | WBbt:0003681 | Certain | |||
| WBbt:0005733 | Certain | ||||
| WBbt:0005772 | Partial | ||||
| Remark | Expressed in the terminal web of the intestinal cells. | ||||
| GO_term | GO:0005911 | ||||
| Subcellular_localization | The C1-GFP expression was also detected in cellular junctions of the pharynx in the early larval developmental stage. | ||||
| Type | Antibody | Double staining experiments with the C1-specific rabbit antibody and the monoclonal antibody MH4, which strongly reacted with recombinant IF proteins A1, A2 and A3 in immunoblots. | |||
| Reporter_gene | |||||
| Pattern | The expression of C1 in the intestinal terminal web and in the hypodermis was also observed in worms transformed with the C1-promoter/cDNA/gfp reporter. | ||||
| These experiments showed coexpression of the C1 and the MH4 antigens in the dorsal and ventral hypodermis of the bean, 1.5-fold and 3-fold embryos, in the larvae and in adults. However, in contrast to the MH4 staining pattern no visible C1 expression was seen in the pharynx and also the intensity of C1 staining in the larval and adult hypodermis was significantly weaker. C1 expression was detected weakly in the intestinal terminal web of the embryos and strongly in the same structure of all larval developmental stages and of the adults. | |||||
| Reference | WBPaper00024590 | ||||
| Transgene | WBTransgene00028596 | ||||
| Antibody_info | WBAntibody00000059 | ||||
| WBAntibody00000796 |
