WormBase Tree Display for Expr_pattern: Expr3453
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| Expr3453 | Expression_of | Gene | WBGene00001932 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001932 | ||
| Expression_data | GO_term | GO:0005634 | |
| GO:0000793 | |||
| Subcellular_localization | In newly fertilized wild-type embryos, GFP::histone appeared on oocyte chromosomes as they completed meiosis. Fluorescence appeared on sperm chromatin during formation of the sperm pronucleus, presumably reflecting the replacement of sperm-specific chromatin proteins by maternal histones. Thereafter, during mitosis GFP::histone was clearly associated with condensed chromosomes, and during interphase it was distributed diffusely throughout the nucleus. | ||
| Type | Reporter_gene | [pie-1::gfp-his-58] translational fusion. | |
| Picture | WBPicture0000008687 | ||
| Remark | In severe gamma-tubulin(RNAi) embryos, chromosomes condensed during pronuclear migration, as in wild-type embryos. After nuclear envelope breakdown, they clustered together loosely in the center of the embryo. At the time when anaphase should have occurred, chromosomes did not segregate; instead they decondensed and formed multiple micronuclei. In moderate RNAi embryos, chromosomes often congressed together but did not organize into a tight metaphase plate. Anaphase was defective, as evidenced by chromosome bridges, poor segregation, and the consequent formation of multiple micronuclei. | ||
| Reference | WBPaper00004785 | ||
| Transgene | WBTransgene00000159 | ||
| WBTransgene00000294 |
