WormBase Tree Display for Expr_pattern: Expr3462

Expr3462 Expression_of: Gene: WBGene00003968
    Reflects_endogenous_expression_of: WBGene00003968
  Expression_data: Life_stage: WBls:0000024
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
      WBls:0000018
      WBls:0000019
      WBls:0000020
      WBls:0000017
      WBls:0000021
    Anatomy_term (28)
    GO_term: GO:0005634
  Subcellular_localization: nuclei
  Type: Antibody
  Pattern: In summary, PEB-1 accumulated in L1 larvae in the nuclei of all hypodermal cells and the epithelial cells lining the rectal lumen. As in the pharynx, PEB-1 was not detectable in neuroblasts or differentiated neurons. PEB-1-expressing cells are not obviously related by cell type or lineage. Rather, one striking common feature of these cells is that they contact the cuticle on the exterior of the worm or lining the pharyngeal or rectal lumen.
    Like in the pharynx, PEB-1 was first detected in the hypodermal nuclei in comma stage embryos (approximately 400 min) and remained detectable until hatching. In 1 1/2-fold stage embryos, the PEB-1 protein was detected in the nuclei of most, if not all, hypodermal cells including hyp5, hyp6, and hyp7 and the H, P, and V cells. After hatching, PEB-1 remained detectable in most hypodermal cells although it decreased in later larvae and was undetectable in adults. In the L1 lateral hypodermis, the PEB-1 protein was detected in H0, H1, H2, V1 to V6, and T, and their anterior and posterior daughters, as well as in the nuclei of the hyp7 syncytium. PEB-1 was also detected in many of the dorsal and ventral hypodermal nuclei in the head and the ventral hypodermal nuclei in the tail. Notably, PEB-1 was not detected in larval P cells. In addition, PEB-1 was not detected in other neuroblasts including Q and T.p.
    PEB-1 was also expressed in cells lining the lumen of the hindgut. In 1 1/2-fold embryos (approximately 420 min), PEB-1 was detected in many nuclei near the posterior of the embryo. These likely include both the posterior hypodermal cells and hindgut cells, although these cells cannot be easily distinguished at this stage. In L1 larvae, PEB-1 was detected in many of the non-neuronal nuclei surrounding the rectal lumen, including K, K', U, F, B, and Y. PEB-1 was not detected in the rectal-intestinal valve or the anal depressor muscle. As in other tissues, the PEB-1 protein remained detectable in the hindgut throughout larval development but became progressively less abundant and undetectable in adults. Importantly, this progressive decrease in PEB-1 expression also occurs in Y, which withdraws from the hypodermis during late larval development to become a neuron.
  Remark: Interestingly, Y is replaced in the hindgut by P12.pa, a descendant of the only P cell in which PEB-1 protein expression was detected in L1 larvae.
    No detailed description on cellular expression patterns in pharynx, try to find those information in Expr838.
  Reference: WBPaper00024643
  Antibody_info: WBAntibody00000344