WormBase Tree Display for Expr_pattern: Expr3464
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| Expr3464 | Expression_of | Gene | WBGene00003169 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003169 | |||
| Expression_data | Anatomy_term | WBbt:0003675 | Partial | |
| WBbt:0005237 | Certain | |||
| GO_term | GO:0030424 | |||
| GO:0055120 | ||||
| Subcellular_localization | A full-length MEC-5 collagen fusion with GFP bound strongly to the processes of the touch receptor neurons in a pattern similar to that of MEC-1 (see Expr3463). Most of the lateral processes had a uniform fluorescence underlying periodic spots of brighter fluorescence. Only these puncta were seen at the ends of the ALM processes. The uniform fluorescence appears as two parallel lines that run along the neuronal process and that are independent of the angle of observation. This pattern suggests that MEC-5::GFP coats the axon. The ventral processes did not appear to have the uniform fluorescence, but had MEC-5::GFP puncta approximately 2 um apart. The puncta differ in intensity, with occasional puncta being much less intense. Although puncta on the lateral processes are spaced on average further apart, sometimes pairs of puncta are as close together as those on the ventral processes. MEC-5::GFP also adhered as puncta to the edge of the muscle cells and to what appear to be the muscle dense bodies. The cells also have large, randomly placed aggregates. | |||
| Type | Reporter_gene | |||
| Picture | WBPicture0000009642 | |||
| WBPicture0000009643 | ||||
| Reference | WBPaper00024622 | |||
| Transgene | WBTransgene00028621 |
