WormBase Tree Display for Expr_pattern: Expr3677
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| Expr3677 | Expression_of | Gene | WBGene00003812 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003812 | |||
| Expression_data | Anatomy_term | WBbt:0005772 | Certain | |
| GO_term | GO:0009925 | |||
| Subcellular_localization | nrf-5::gfp fluorescence was concentrated at the basal membrane of the intestine. Based on its similarity to secreted lipid binding proteins and the fact that it contains an N-terminal signal sequence, NRF-5 probably is secreted. The concentration of nrf-5::gfp fluorescence at the basal membrane of the intestine suggests that NRF-5 protein is secreted from the intestine into the pseudocoelomic fluid. GFP fluorescence was not observed in the pseudocoelomic fluid itself, however one possibility is that the level of secreted nrf-5::gfp is too low to detect in this manner, particularly against the background of fluorescence from the intestine. | |||
| Type | Reporter_gene | |||
| Pattern | GFP fluorescence was observed throughout the entire intestine of transgenic animals expressing this nrf-5::gfp fusion. No GFP expression was observed in any other tissue. Furthermore, the nrf-5::gfp fusion rescued the Nrf and Peg phenotypes of nrf-5 mutants. | |||
| Picture | WBPicture0000010915 | |||
| Reference | WBPaper00026767 | |||
| Transgene | WBTransgene00028810 |
