WormBase Tree Display for Expr_pattern: Expr3766
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Expr3766 | Expression_of | Gene | WBGene00004750 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00004750 | ||
Expression_data | Life_stage | WBls:0000003 | |
GO_term | GO:0005634 | ||
Subcellular_localization | SEA-1 accumulates in nuclei of young embryos, as indicated by immunofluorescence experiments with anti-SEA-1 antibodies. | ||
Type | Antibody | ||
Picture | WBPicture0000009981 | ||
Remark | Antibodies raised to an N-terminal peptide of SEA-1 recognized a 43 kDa band in Western blots of wild-type embryonic extract, in agreement with the predicted size of SEA-1. This band is more abundant in extract made from embryos carrying the chromosomally integrated yIs61[sea-1(+)] array, which has multiple copies of sea-1(+), and is missing from sea-1(y356) mutant embryonic extract, confirming the specificity of the antibody, the overexpression of SEA-1 from the yIs61[sea-1(+)] array, and the loss of SEA-1 in the mutant. Consistent with the Western blot, SEA-1 accumulates to higher levels in overexpressing yIs61[sea-1(+)] embryos and is absent from sea-1(y356) mutant embryos | ||
Reference | WBPaper00026779 | ||
Antibody_info | WBAntibody00000877 |