WormBase Tree Display for Expr_pattern: Expr3783
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| Expr3783 | Expression_of | Gene | WBGene00006779 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006779 | |||
| Expression_data | Anatomy_term | WBbt:0005828 | Certain | |
| WBbt:0006797 | Certain | |||
| GO_term | GO:0005938 | |||
| GO:0031941 | ||||
| Subcellular_localization | To evaluate the subcellular distribution, a z-series was generated through the gonad and processed the images with deconvolution software. Phosphorylated T284 (pT284) UNC-43 co-localizes with F-actin at the oocyte cortex and it is enriched at the oocyte/oocyte interface, similar to the distribution of GFP::NMR-1 and VAB-1::GFP(see Expr3782). CaMKII-pT staining is also detected at the sheath/oocyte interface of wild-type gonads. The CaMKII-pT staining pattern contrasts with that of MAPK-YT, which is uniformly distributed throughout the oocyte cytoplasm. In wild-type gonads, it is difficult to determine whether UNC-43 is expressed in sheath cells, owing to their close proximity to oocytes and the thinness of the sheath. To test whether pT284 UNC-43 is in sheath cells, fem-3(q20gf) mutant gonads were stained with anti-CaMKII-pT. When grown at the restrictive temperature, fem-3(q20gf) mutant gonads contain sperm and sheath cells, but not oocytes. CaMKII-pT stains the somatic cells surrounding sperm in fem-3(q20gf) gonads. These results indicate that UNC-43 is phosphorylated in sheath cells and in oocytes when sperm are present. In oocytes, pT284 UNC-43 co-localizes with F-actin at the cortex. | |||
| Type | Antibody | Antibody against rat CaMKII (CaMKII-pT). To investigate unc-43 expression, gonads were incubated with an antibody that recognizes phosphorylated (pT286) rat CaMKII (CaMKII-pT). The rat sequence used to generate CaMKII-pT is highly conserved in UNC-43, including the phosphorylated threonine. The CaMKII-pT antibody stains oocytes of wild-type gonads, but not of unc-43(n1186) null and unc-43(e408) loss-of-function gonads. Therefore, CaMKII-pT specifically recognizes the unc-43 gene product. | ||
| Reference | WBPaper00026932 |
