WormBase Tree Display for Expr_pattern: Expr4838
expand all nodes | collapse all nodes | view schema
| Expr4838 | Expression_of | Gene | WBGene00018304 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00018304 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| GO_term | GO:0005604 | |||
| Subcellular_localization | Agrin was detected in the basal lamina around the pharynx procorpus and anterior bulb. Posterior bulb staining was weaker possibly due to poor antibody penetration. | |||
| Type | Antibody | To localize the agrin protein, the worms were co-stained with the monoclonal antibody pool against C. elegans agrin and with polyclonal anti-rim antibody, recognizing a pre-synaptic marker prominent in the nerve ring. | ||
| Pattern | The major site of agrin expression was around the pharynx and the staining was particularly enriched in the anterior part. The posterior bulb was labeled more weakly correlating with the fainter GFP reporter expression in the posterior part. Polyclonal antiserum staining resulted in the same staining pattern in wild type worms of different developmental stages. Young larvae (L1) generally showed stronger agrin staining compared to young adults. In addition to the pharynx staining in the wild type worms, the polyclonal antiserum stained the gut lumen both in the wild type worms as well as in the agrin mutants, but not when preimmune serum was used. The staining of the lumen of the gut represents an unrelated cross-reactivity of the antiserum, possibly corresponding to the background bands detected on the western blots. | |||
| Picture | WBPicture0000007306 | |||
| WBPicture0000007329 | ||||
| Remark | Picture: Figure 8 C and D. | |||
| Reference | WBPaper00030960 | |||
| Antibody_info | WBAntibody00001294 | |||
| WBAntibody00001295 |
