WormBase Tree Display for Expr_pattern: Expr606
expand all nodes | collapse all nodes | view schema
| Expr606 | Expression_of | Gene | WBGene00001953 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001953 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| Anatomy_term (36) | |||
| Type | Antibody | Expression vector pRSETA was used to express CeTwist protein in E.coli BL21 (lys-S) cells as a His-tagged fusion protein. CeTwist-His protein was purified with Ni affinity column in denaturing conditions. Polyclonal antibody was produced in mice. | |
| Pattern | Immuno fluorescence staining with antisera to ceTwist indicated similar expression pattern to reporter fusion. CeTwist first detected in L1 in defecation-associated muscles and in small number of neuron-like cells in the head. In later larvae, CeTwist was detected in SMs and their descendants. Expression in M and its descendants prior to to the SM stage was detected with reporter fusion but not with antibody. Expression in mature coelomocytes was also only detected with reporter fusions. | ||
| Remark | CeTwist means hlh-8 here. See Expr607 for the pattern of the same locus. | ||
| This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). | |||
| Reference | WBPaper00003174 | ||
| Antibody_info | WBAntibody00000188 |
