WormBase Tree Display for Expr_pattern: Expr838
expand all nodes | collapse all nodes | view schema
| Expr838 | Expression_of | Gene | WBGene00003968 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003968 | ||
| Expression_data | Life_stage | WBls:0000002 | |
| Anatomy_term (12) | |||
| GO_term | GO:0005634 | ||
| Subcellular_localization | PEB-1 protein was strongly detected in the nuclei. | ||
| Type | Antibody | Rabbit polyclonal antiserum was raised against full-length MBP PEB-1. | |
| Pattern | In embryos, PEB-1 protein was strongly detected in the nuclei of many cells in the developing pharynx. Staining was first detected at the comma stage. Some pharyngeal cells do not express PEB-1, observed a cluster of nuclei that do not contain PEB-1 near the center of the developing pharynx, a region containing many of the pharyngeal neurons. Strong pharyngeal staining persisted through the remainder of embryogenesis. PEB-1 staining was also observed outside the pharynx in several cells near the rectum and in the tail and at lower level in hypodermal nuclei. In early larvae, PEB-1 protein is easily detectable in the pharynx, as well as in a subset of nonpharyngeal. Consistently observed PEB-1 protein in the nuclei of all pharyngeal muscles, marginal cells, epithelial cells, gland cells, and pharyngeal-intestinal valve cells. Notably, authors did not observe PEB-1 staining in any pharyngeal neurons. Pharyngeal PEB-1 staining decreased in late larvae until it became undetectable in adults. PEB-1 protein expression was also observed in some tissues outside the pharynx in larvae and adults. In general, the nonpharyngeal expression was weaker than expression in the pharynx, and it appeared dynamic during the worm life cycle. In larvae, PEB-1 antibody staining was reproducibly observed in several cells near the rectum and in hypodermal nuclei in the tail and in the developing vulva and surrounding cells. Authors also observed protein expression in several nuclei in the head and tail that were provisionally identified as hypodermal nuclei. In older larvae and adults, the rectal and vulval expression decreased, but PEB-1 staining was visible in germ-line cells. This expression is consistent with Northern blots indicating that peb-1 mRNA is expressed in the germ line. | ||
| Remark | See Expr837, 839, 840 for Expr_pattern of the same locus. | ||
| Reference | WBPaper00004526 | ||
| Antibody_info | WBAntibody00000344 |
