WormBase Tree Display for Expr_pattern: Expr8560
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| Expr8560 | Expression_of | Gene | WBGene00002024 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002024 | |||
| Expression_data | Life_stage | WBls:0000038 | ||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0004510 | Certain | ||
| WBbt:0004511 | Certain | |||
| WBbt:0005319 | Certain | |||
| WBbt:0005767 | Certain | |||
| WBbt:0005813 | Certain | |||
| WBbt:0006748 | Certain | |||
| WBbt:0006789 | Certain | |||
| WBbt:0006791 | Certain | |||
| GO_term | GO:0005911 | |||
| Subcellular_localization | In the vulval region, HSP43 was localized in the adherens junctions of vulval epithelial cells, In all post-embryonic stages, HSP43 staining was observed in a very regular punctate pattern over body-wall muscle, the columns of labelled spots running circumferentially along the muscle quadrant and the signal being more intense where the muscle cells contact each other. Individual bands could be resolved as doublets separated by a narrow space. | |||
| Type | Antibody | |||
| Pattern | At the same developmental stages, HSP43 staining was seen in desmosomes of cells which make up the spermathecal cage and the spermathecal valve. L2 larvae, which lack vulva and spermatheca, showed prominent HSP43 staining anteriorly in the pharyngealintestinal valve, a structure made up of six cells coupled by a system of desmosomes, and posteriorly in a discreet region probably corresponding to the intestinalrectal valve. | |||
| In both control and heat-shocked adult and L4 larvae a low level of staining was seen throughout the body, together with very strong, specific, staining in the vulva and spermatheca. In the vulval region, HSP43 was localized in the adherens junctions of vulval epithelial cells, but absent from vulval muscle. Images taken in different focal planes of the vulval region revealed HSP43 staining in the utse cells and in the uv1 cells. | ||||
| Picture | WBPicture0000011725 | |||
| Remark | Picture: Figure 2. | |||
| This pattern appears identical with that reported for monoclonal antibody MH5, which labels fibrous organelles containing hemidesmosomes associated with the hypodermis, where it contacts the muscle cells. Similar patterns, but differing in some details, were noted with monoclonal antibodies MH4 and MH46. The specificity of the antiserum for HSP43 is demonstrated by the loss of signal when the antibody is pre-incubated with excess pure recombinant HSP43. | ||||
| Reference | WBPaper00004245 | |||
| Antibody_info | WBAntibody00001840 |
