WormBase Tree Display for Expr_pattern: Expr8623
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| Expr8623 | Expression_of | Gene | WBGene00014115 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00014115 | |||
| Expression_data | Anatomy_term | WBbt:0004575 | Certain | |
| WBbt:0004576 | Certain | |||
| WBbt:0005784 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0043186 | ||||
| Subcellular_localization | Subcellularly, GLD-4 localizes also to cytoplasmic structures that are nuclear envelope-associated and the staining pattern is reminiscent of P granules. Indeed, GLD-4 overlaps in colocalization experiments with PGL-1, a molecular marker for P granules. The staining pattern was observed with both C-terminal anti-GLD-4 antibodies and is reduced to background signals in the gld-4(ef15) allele. The P granule association of GLD-4 was less apparent during pachytene, became obvious again in maturing oocytes, and was most prominently visible in developing embryos. | |||
| Type | Antibody | |||
| Pattern | Adult hermaphrodites and males expressed more GLD-4 than animals containing no germline. By immunocytochemistry, GLD-4 was found to be cytoplasmic in the hermaphrodite germline and primordial germ cells throughout embryogenesis. In the late embryo, GLD-4 expression became limited to the germ cell lineage and its localization correlated perfectly with embryonic P granules. GLD-4 was detected throughout embryonic development in the entire P-cell lineage, which gives rise to the two primordial germ cells Z2 and Z3. | |||
| Picture | WBPicture0000008616 | |||
| Remark | Picture: Fig 2. | |||
| Reference | WBPaper00033036 | |||
| Antibody_info | WBAntibody00001897 | |||
| WBAntibody00001898 | ||||
| WBAntibody00001899 |
