WormBase Tree Display for Expr_pattern: Expr8634
expand all nodes | collapse all nodes | view schema
| Expr8634 | Expression_of | Gene | WBGene00043056 | |
|---|---|---|---|---|
| WBGene00017742 | ||||
| WBGene00011614 | ||||
| WBGene00021132 | ||||
| Reflects_endogenous_expression_of | WBGene00011614 | |||
| WBGene00017742 | ||||
| WBGene00021132 | ||||
| WBGene00043056 | ||||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0005175 | Certain | ||
| WBbt:0005812 | Certain | |||
| WBbt:0006751 | Partial | |||
| WBbt:0006797 | Certain | |||
| GO_term | GO:0005737 | |||
| Subcellular_localization | cytoplasm | |||
| Type | In_situ | |||
| Pattern | A different expression pattern of CeNF-Y genes was observed in infertile worms (12 days after L1 stage). The positivity in the gonads disappeared, while CeNF-YB and CeNF-YC expression decreased in the pharingeal secretory cells and the neurons. | |||
| Apart from the gonads and developing embryos, the CeNF-Y mRNAs were detected in other tissue from C. elegans. In 6-day-old worms, CeNF-YB and CeNF-YC, but not CeNF-YA1 nor CeNF-YA2, were expressed in the cytoplasm of the secretory cells located in the pharyngeal terminal bulb wall, in the neuron cell bodies of the small ganglia surrounding the pharynx and in the neurons running anteriorly to the sensory organs in the animal's head. NF-YC was also found in the excretory cell. Subunits were not expressed in any other organs, such as the intestine, the hypodermis and body wall muscle surrounding the pseudocoelomic space. | ||||
| The in situ hybridization procedure revealed the expression of CeNF-Y gene subunits in adult, fertile, hermaphrodite (6 days after L1 stage) C. elegans. High mRNA expression of all the subunits was evident in various parts of the gonads with differing degrees of intensity. In particular, positivity increased in oocytes from the distal to the proximal arm of the gonad in function of the stage of maturation. The cytoplasm of the syncytial ovaries and immature oocytes at the beginning of the proximal arm of the gonad were weakly positive, while oocytes, which increased in size along the oviduct toward the spermatheca, were strongly positive. Fertilized oocytes present in the uterus were strongly positive. In the late embryo stage, the expression of CeNF-Y mRNAs did not appear to be uniformly distributed, and presented an asymmetrical organization with anterior-posterior polarity. | ||||
| Remark | Picture: Fig 2, 3, 4. | |||
| The controls of the in situ hybridiza tion reactions showed negative results. | ||||
| Reference | WBPaper00024952 |
