WormBase Tree Display for Expr_pattern: Expr8801
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| Expr8801 | Expression_of | Gene | WBGene00002855 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002855 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000003 | ||||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000032 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005175 | Certain | |||
| WBbt:0005319 | Certain | |||
| WBbt:0005337 | Certain | |||
| WBbt:0005733 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005813 | Certain | |||
| GO_term | GO:0005739 | |||
| Subcellular_localization | Subcellular localization of LET-721::GFP is not uniformly dispersed throughout each tissue, rather, it is distributed in an intracellular punctate pattern of reporter expression, indicating mitochondrial localization of the LET-721::GFP construct. To verify that the punctate pattern of expression was a consequence of mitochondrial localization, a GFP reporter strain lacking the mitochondrial localization sequence of LET-721 was examined. This transgenic strain contained a promoter::GFP fusion construct, using the same 5 gene-upstream non-coding region as the previous protein fusion (1,343 bp sequence upstream from the start codon of let-721), but lacking the protein coding sequence for let-721. The let-721-promoter::GFP (BC10569) construct drove expression of GFP in the same tissue types as the translational reporter fusion (pharynx, intestines, muscle, hypoderm, unassigned neurons and spermatheca). However, the let-721-promoter::GFP strain did not exhibit the punctate expression associated with mitochondrial localization, but rather a uniform distribution of fluorescence throughout cells with a concentration of signal localizing to the nucleus due to the influence of the 5' nuclear localization sequence. Along with similar intracellular patterns observed by another group's study of a different mitochondrial protein, the punctate pattern of expression is likely a consequence of the LET-721::GFP protein fusion localizing to the mitochondria. | |||
| Type | Reporter_gene | |||
| Pattern | The presence of LET-721::GFP was observed during all developmental stages: embryo, larvae and adult. The LET-721::GFP was also expressed in dauer larvae, an alternate larval stage C. elegans enter under stress conditions. Observation of the spatial expression pattern revealed that LET-721 is found in a many of tissues including: pharynx, body wall muscle, hypoderm, intestine and somatic gonad. In addition to these tissues, sex-specific expression was observed in hermaphrodites, which express LET-721::GFP in the spermatheca, while males express LET-721 in the vas deferens. | |||
| Remark | Picture: Fig 2. | |||
| Reference | WBPaper00035219 | |||
| Transgene | WBTransgene00030726 |
