WormBase Tree Display for Expr_pattern: Expr889

Expr889 Expression_of: Gene: WBGene00002249
    Reflects_endogenous_expression_of: WBGene00002249
  Homol: Homol_homol: ZK353:Expr
  Expression_data: Life_stage: WBls:0000002
    Anatomy_term: WBbt:0003681 Certain
      WBbt:0005255 Certain
      WBbt:0005772 Certain
      WBbt:0005800 Uncertain
        Remark: Three paired rectal nuclei are stained.
    GO_term: GO:0005634
  Subcellular_localization: nuclei
  Type: Reporter_gene
  Pattern: In all larval and adult stages stained cells are intestinal with some variation in the staining pattern between stages. Thus, in all stages, paired nuclei posterior to the pharyngealintestinal valve are stained and either paired or single nuclei along the length of the intestine. In adult worms, three paired rectal nuclei are stained which are not all seen in larval stages. That stained nuclei are in intestinal cells is indicated by distribution, size, shape and topology. Whereas LAP-1-lacZ transgenes are expressed along the length of the gut but not in the buccal cavity or pharynx, Indirect Fluorescent Antibody Tests using an antiserum specific for the protein show staining most intensely in the buccal cavity and rectal epithelium with staining also along the lining of the posterior pharyngeal bulb and in the in the anterior gut.
  Remark: This staining pattern was seen in all stages of wild type nematodes and was absent in deletion mutants and using pre-immune sera.
  Reference: WBPaper00004658
  Transgene: WBTransgene00030774