WormBase Tree Display for Expr_pattern: Expr9076

Expr9076 Expression_of: Gene: WBGene00009098
    Reflects_endogenous_expression_of: WBGene00009098
  Expression_data: Anatomy_term: WBbt:0003681 Certain
      WBbt:0005319 Certain
      WBbt:0005753 Certain
      WBbt:0005772 Certain
      WBbt:0005813 Certain
      WBbt:0005828 Certain
      WBbt:0006749 Certain
    GO_term: GO:0016323
      GO:0016324
      GO:0010008
      GO:0031901
      GO:0055038
  Subcellular_localization: In the C. elegans intestine, RFP-RAB-10 and GFP-RAB-8 colocalized extensively. Closer examination showed that EHBP-1 colocalized with RAB-10 and RAB-8 on punctate endosomal membranes. EHBP-1 also localized to tubular elements associated with the puncta and the plasma membrane. EHBP-1-GFP also occasionally overlapped with the early endosome marker RFP-RAB-5, but the EHBP-1-GFP labeled structures more often localized near RFP-RAB-5 puncta rather than overlapping with them directly. EHBP-1-GFP was not found to colocalize with the apical recycling marker RFP-RAB-11 or the Golgi marker MANS-RFP. Taken together these results indicate that EHBP-1 is enriched on endosomes.
    The EHBP-1-GFP fusion protein was localized in a punctate or tubular pattern in most tissues. In the intestine, EHBP-1-GFP was localized to both the apical and basolateral plasma membranes. Near basolateral membranes, EHBP-1-GFP labeled distinct cytoplasmic puncta resembling endosomes ranging in size from 0.5 to 1.0 um. In addition to punctate structures, EHBP-1-GFP also labeled tubular structures close to the basolateral plasma membrane of the intestine.
  Type: Reporter_gene
  Pattern: EHBP-1-GFP fusion proteins were expressed in all somatic C. elegans tissues, including the pharynx and nerve ring, body-wall muscles, seam cells, intestine, oviduct sheath cell and spermatheca.
  Picture: WBPicture0000008884
    WBPicture0000008885
  Remark: Picture: Fig 2, Fig 3, Fig S4.
  Reference: WBPaper00036404
  Transgene: WBTransgene00030890