WormBase Tree Display for Expr_pattern: Expr9595
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| Expr9595 | Expression_of | Gene | WBGene00007490 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00007490 | ||
| Expression_data | Anatomy_term (17) | ||
| GO_term | GO:0035869 | ||
| Subcellular_localization | The mCherry-tagged NPHP-8 was only observed at the distal end of dendrites of amphid and phasmid neurons in hermaphrodites. The NPHP-8 protein specifically co-localized with NPHP-4 at the transition zones at the bases of the cilia. Little or no fluorescence was found in the ciliary axonemes. | ||
| Type | Reporter_gene | [NPHP-8::SL2-GFP], [NPHP-8::mCherry], translational fusion. Two nphp-8 gene fragments were obtained using PCR. A 5.9 kb fragment containing the 3.9 kb promoter and the genomic sequence (exons and introns) up to exon 6 and an- other 3.0 kb fragment containing all of the exons downstream of exon 6 were amplified from the total cDNA library. These two sequences were inserted into vector pXTL115 in the proper order to generate the translational fusion pXTL123 (NPHP-8::mCherry). To create the SL2 expression vector pXTL162 (NPHP-8::SL2-GFP), the two fragments (the 3.0 kb nphp-8 fragment with a stop codon) were cloned into the pJG7 vector. | |
| Pattern | Strong NPHP-8 expression was found in a subset of ciliated sensory neurons in hermaphrodites. The expression of nphp-8 overlapped with but was not limited to, DiI loaded neurons, including six neurons in the head (ASI, ASH, AWB, ASJ, ASK, and ADL) and two neurons in the tail (PHA and PHB).To determine the subcellular localization of NPHP-8 in C. elegans, an expression construct containing NPHP-8 fused to red fluorescent protein mCherry, driven by its own promoter, was created. The mCherry-tagged NPHP-8 was only observed at the distal end of dendrites of amphid and phasmid neurons in hermaphrodites. The NPHP-8 protein specifically co-localized with NPHP-4 at the transition zones at the bases of the cilia. Little or no fluorescence was found in the ciliary axonemes. | ||
| Picture | WBPicture0000012545 | ||
| Reference | WBPaper00039789 | ||
| Transgene | WBTransgene00014519 |
