WormBase Tree Display for Expr_pattern: Expr966
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| Expr966 | Expression_of | Gene | WBGene00003919 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003919 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| WBls:0000057 | |||
| Anatomy_term | WBbt:0005175 | ||
| GO_term | GO:0005737 | ||
| GO:0005938 | |||
| Subcellular_localization | At the actin-rich boundaries in the gonads of adult hermaphrodites between nuclei in the syncitial region. In oocytes, the protein is present in the cytoplasm, is excluded by nuclei and is faintly present at the cell periphery. | ||
| Type | Antibody | Indirect immunofluorescence. Polyclonal antibodies (rabbit) to the amino-terminal portion of PAR-4 including amino acids 6-169, preceding the kinase domain. Antibodies from both rabbits recognize an 80 kDa protein in wild-type C. elegans extracts that is absent in par-4(it75) mutant worms. | |
| Pattern | PAR-4 protein is apparent in the gonads of adult hermaphrodites and in oocytes. 1-cell embryos show cytoplasmic staining and faint cortical staining. No asymmetry in the distribution of PAR-4 protein in the cytoplasm or at the cell periphery was observed. Cortical staining is stronger at the time of first cleavage, with prominent PAR-4 staining of the cleavage furrow. The cortical staining remains at high levels at the 2-cell and 4-cell stages and continues to be present in all blastomeres through early development. Staining of late stage embryos is reduced, but the germline precursor cells, Z2 and Z3, show somewhat stronger cortical staining than other cells. | ||
| Picture | WBPicture0000008044 | ||
| WBPicture0000008045 | |||
| Reference | WBPaper00003944 | ||
| Antibody_info | WBAntibody00000269 |
