WormBase Tree Display for Expr_pattern: Expr9885
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| Expr9885 | Expression_of | Gene | WBGene00000858 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000858 | ||||
| Expression_data | Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005733 | Certain | ||||
| WBbt:0005753 | Certain | ||||
| WBbt:0005772 | Partial | ||||
| Remark | Detected along the boundary between the intestine and muscle or hypodermis. | ||||
| Type | Reporter_gene | [cwn-2p::CWN-2::Venus] translational fusion. cwn-2p::CWN-2::Venus was constructed from PCR fragments containing its promoter region (6.1 kb), and the entire coding region was amplified by PCR from the fosmid WRM0622bE06 inserted into a pPD95.75::wVenus derived from pPD95.75 (a gift from A. Fire) and containing the Venus gene optimized for C. elegans codon usage in place of the GFP gene. --precise ends. | |||
| Pattern | We detected puncta of cwn-2p::CWN-2::Venus mostly around the pharynx. We detected these puncta on the hypodermis, including the seam cells, suggesting its diffusion from the pharyngeal region. Although it was also reported that the cwn-2 promoter is active in the intestine, albeit weaker than in the pharynx, we detected cwn-2p::CWN-2::Venus puncta only in the anterior region, along the boundary between the intestine and muscle or hypodermis. These observations indicate that CWN-2 is mostly distributed to the anterior side of the animal. | ||||
| Picture | WBPicture0000012591 | ||||
| Reference | WBPaper00040343 | ||||
| Transgene | WBTransgene00014762 |
