WormBase Tree Display for Antibody: WBAntibody00003035
expand all nodes | collapse all nodes | view schema
| WBAntibody00003035 | Summary | Polyclonal antibodies against an N-terminal fragment of UNC-16 (residues 1-506) were generated as follows: GST::UNC-16(1-506) was expressed from pGEX6P-1 in bacteria, purified as described below, and injected into rabbits (GeneCust). For affinity purifi- cation of the antibodies, the GST fusion protein was cleaved using Prescission protease, and UNC-16(1-506) was covalently coupled to a 1-ml HiTrap N-hydroxysuccinimide column (GE Healthcare). | ||
|---|---|---|---|---|
| Public_name | [WBPaper00064276]::anti-UNC-16 | |||
| Gene | WBGene00006755 | |||
| Isolation | Original_publication | WBPaper00064276 | ||
| Location | GCP | |||
| Clonality | Polyclonal | |||
| Antigen | Peptide | residues 1-506 | ||
| Animal | Rabbit | |||
| Reference | WBPaper00064276 |
