WormBase Tree Display for Construct: WBCnstr00000171
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| WBCnstr00000171 | Summary | [mab-23::gfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00003114 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | mab-23::gfp reporter (EM#308) is based on the minimal 9.5-kb rescuing region in C32C4 and consists of mab-23-coding sequence flanked by 5.3 kb of 5' promoter and 2.8 kb of 3' non-coding sequence with GFP inserted in frame immediately 5' of the mab-23 termination codon. EM#308 was generated by inserting a 7-kb fragment (position 493811939 in C32C4) and a 2.8-kb fragment (position 1193914494 in C32C4) into the BssHKpnI and NheIStuI sites of pPD117.75, respectively. bxEx83 and bxEx84 were generated by coinjection of linearized EM#308 (1 ng/L), N2 (50 ng/L) and pBX-1[pha-1(+)] (1 ng/L) DNA into a pha-1(e2123ts); him-5(e1490) strain. --precise ends. Phenotype: Low levels of reporter expression, generated by noncomplex EM#308-containing arrays, rescue the mab-23 phenotypes. However, high levels of reporter expression, generated by complex arrays bxEx83 or bxEx84, resulted in partial rescue of gonad and ray phenotypes. As the presence of bxEx83 or bxEx84 in a wild-type background also caused a low frequency of mab-23 gonad phenotype (20% of males) and ray 3 loss (15% of male tail sides, compared with 5% in nontransgenic males). | ||
| Used_for | Transgene_construct | WBTransgene00000171 | |
| Reference | WBPaper00005463 |
