WormBase Tree Display for Construct: WBCnstr00000991
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WBCnstr00000991 | Summary | [daf-5::gfp] | |
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Driven_by_gene | WBGene00000901 | ||
Fusion_reporter | GFP | ||
Construction_summary | A 3855 bp fragment of W01G7.1 genomic DNA containing 2563 bp of 5' sequence, exon 1, and part of exon 2 was cloned into the GFP expression vector pPD95.75 (without a nuclear localization signal) and coinjected into the germline of N2 with the dominant rol-6 transformation marker to produce a line of genotype mEx127[daf-5:gfp rol-6(su1006)]. This extrachromosomal transgene was integrated into chromosomal DNA by exposure to gamma radiation. | ||
Used_for | Transgene_construct | WBTransgene00001000 | |
Reference | WBPaper00006425 |