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WormBase Tree Display for Construct: WBCnstr00000991

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Name Class

WBCnstr00000991Summary[daf-5::gfp]
Driven_by_geneWBGene00000901
Fusion_reporterGFP
Construction_summaryA 3855 bp fragment of W01G7.1 genomic DNA containing 2563 bp of 5' sequence, exon 1, and part of exon 2 was cloned into the GFP expression vector pPD95.75 (without a nuclear localization signal) and coinjected into the germline of N2 with the dominant rol-6 transformation marker to produce a line of genotype mEx127[daf-5:gfp rol-6(su1006)]. This extrachromosomal transgene was integrated into chromosomal DNA by exposure to gamma radiation.
Used_forTransgene_constructWBTransgene00001000
ReferenceWBPaper00006425