WormBase Tree Display for Construct: WBCnstr00005103
expand all nodes | collapse all nodes | view schema
| WBCnstr00005103 | Summary | [dpy-30::sdc-2] | |
|---|---|---|---|
| Driven_by_gene | WBGene00001088 | ||
| Gene | WBGene00004746 | ||
| Construction_summary | To create the dpy-30::sdc-2 transgene, a BglII site we introduced at the fourth codon of sdc-2 and an 11-kb BglIIKpnI sdc-2 fragment was subcloned into the Bcl I and Kpn I sites of pTY647, a plasmid containing the dpy-30 minimal rescuing region. The resulting transgene, pTY975, included the dpy-30 promoter, the first three codons of dpy-30, a leucine codon, and the entire sdc-2 structural gene beginning with its fifth codon. pTY975 was then injected with the transformation marker p76-16B [unc-76(+)]into him-8(e1489); unc-76 hermaphrodites and established transmitting lines. Extrachromosomal arrays bearing pTY975 were stably integrated into the genome by Gamma-irradiation for 15 min. Five independent integrated lines were isolated, including yIs29 on X and yIs30. | ||
| Used_for | Transgene_construct | WBTransgene00005126 | |
| Reference | WBPaper00003557 |
