WormBase Tree Display for Construct: WBCnstr00006524
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| WBCnstr00006524 | Summary | [unc-119p::GFP::DAF-16] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006843 | ||
| Gene | WBGene00000912 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | To generate this daf-16 tissue-specific promoter fusion, HindIII and SnaB1 sites were introduced immediately 5' to the first ATG by PCR amplification of the daf-16 cDNA construct (yk13f11, identified by Y. Kohara) using an inverse PCR strategy (HindIII is more proximal to the ATG). A HindIII GFP fragment from the L2911 plasmid (gift from A. Fire) was inserted into the HindIII site to generate an in-frame fusion of GFP immediately upstream of the N terminus of DAF-16a (pNL205). The SnaB1 site was used to insert the pan-neuronal Punc-119 promoter, which contains 2.2 kb upstream sequence of the unc-119 gene (Maduro and Pilgrim, 1995), subcloned into pNL205 using blunt-ended XbaI/BamHI flanking sites, (pNL206) (Libina et al. 2003). | ||
| Used_for | Transgene_construct | WBTransgene00006681 | |
| Reference | WBPaper00006226 | ||
| WBPaper00035871 | |||
| WBPaper00042119 |
