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WormBase Tree Display for Construct: WBCnstr00006614

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Name Class

WBCnstr00006614Summary[Punc-129::wrk-1, Punc-47::wrk-1]
Driven_by_geneWBGene00006783
WBGene00006852
GeneWBGene00006942
Construction_summarypTB118 was generated by cloning a Cfr9I/Bsp120I fragment from pTB111 into pTB116 (punc-129::gfp). pTB116 was generated by cloning a PCR fragment amplified from genomic DNA via oTB155 5'-AAACTGCAGAAACATGATATCGACGGAC-3' and oTB156 5'-TTTCCCGGGTTTCTTGCTTGCTCTTCC-3' into a XmaI/PstI fragment from pPD95.75. pTB112 was generated by cloning a XmaI/ApaI fragment from pTB111 into punc47::gfp. punc-47::gfp was generated by cloning the promoter sequence of the unc-47 gene spanning from 21190 bp to 27 bp relative to its ATG into pPD95.75. pTB111 was generated by cloning two PCR fragments into a pPD95.75 EcoRI/ PstI fragment: (1) oTB140 5'-AAACTGCAGCTTTAAAAAGTGTCAGACGATC-3' and oTB142 5' -GACGACTTCTGTGTTCTTCCTCC-3'. pTB111 was generated by cloning two PCR fragments into a pPD95.75 EcoRI/ PstI fragment: (1) oTB140 5'-AAACTGCAGCTTTAAAAAGTGTCAGACGATC-3' and oTB142 5'-GACGACTTCTGTGTTCTTCCTCC-3' were used to amplify the same promoter sequence present #1; (2) oTB143 5'-AAACCCGGGATGATGAAATTGGTTCTGCTG-3' and oTB136 5'-TTTGAATTCTTAAAATAAAAGATACATTGTGAC-3' were used to amplify the wrk-1b cDNA from yk498f3.
Used_forTransgene_constructWBTransgene00006774
ReferenceWBPaper00028566