WormBase Tree Display for Construct: WBCnstr00007997
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| WBCnstr00007997 | Summary | [tax-4p::CHB-3::GFP] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006526 | ||
| Gene | WBGene00000917 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | For the chb-3 cDNA expression analysis, most of the chb-3 cDNA was obtained from the yk280C9 cDNA clone (provided by Yuji Kohara). The 5' region of the first exon was obtained using 5' RACE (5'-RACE system, Invitrogen), and fused to the yk280C9 cDNA to create a full-length cDNA. A BamHI site just upstream of the initial ATG codon was artificially created using PCRs. The stop codon was changed to a BalI site using PCRs. The cDNA was inserted in-frame into pPD95.75 (GFP expression vector; a gift from Andy Fire) using the BamHI and BalI sites (CHB-3::GFP vector). Upstream promoter sequence from tax-4 (approximately 3 kb) was obtained using PCRs and inserted upstream of the chb-3 coding region of the CHB-3::GFP vector to create tax-4p::CHB-3::GFP. | ||
| Used_for | Transgene_construct | WBTransgene00008287 | |
| Reference | WBPaper00037841 |
