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WormBase Tree Display for Construct: WBCnstr00010476

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Name Class

WBCnstr00010476Other_nameExpr1835_Ex
Summary[dpy-19::gfp]
Driven_by_geneWBGene00001078
GeneWBGene00001078
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[dpy-19::gfp] translational fusions containing 4.4 kb of upstream sequence, the entire dpy-19-coding sequence (with introns) and 1.0 kb of downstream sequence. For the N-terminal GFP-tagged construct, site-directed mutagenesis was used to insert a unique BamHI site after the second codon of dpy-19 and inserted F64L S65T GFP with 3' introns. For the C-terminal GFP-tagged construct, site-directed mutagenesis was used to make a silent change that introduced a BglII restriction site at the 3rd from last codon of dpy-19. We then inserted the coding sequence for S65T GFP with 3 introns (from pPD95.85).
Used_forTransgene_constructWBTransgene00027711
ReferenceWBPaper00004437