WormBase Tree Display for Construct: WBCnstr00010476
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WBCnstr00010476 | Other_name | Expr1835_Ex | |
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Summary | [dpy-19::gfp] | ||
Driven_by_gene | WBGene00001078 | ||
Gene | WBGene00001078 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [dpy-19::gfp] translational fusions containing 4.4 kb of upstream sequence, the entire dpy-19-coding sequence (with introns) and 1.0 kb of downstream sequence. For the N-terminal GFP-tagged construct, site-directed mutagenesis was used to insert a unique BamHI site after the second codon of dpy-19 and inserted F64L S65T GFP with 3' introns. For the C-terminal GFP-tagged construct, site-directed mutagenesis was used to make a silent change that introduced a BglII restriction site at the 3rd from last codon of dpy-19. We then inserted the coding sequence for S65T GFP with 3 introns (from pPD95.85). | ||
Used_for | Transgene_construct | WBTransgene00027711 | |
Reference | WBPaper00004437 |