WormBase Tree Display for Construct: WBCnstr00010744
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| WBCnstr00010744 | Other_name | Expr2271_Ex | |
|---|---|---|---|
| Summary | [dpy-22::gfp] | ||
| Driven_by_gene | WBGene00001081 | ||
| Gene | WBGene00001081 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [dpy-22::gfp] translational fusion. dpy-22 fragments were amplified by PCR from F47A4 using the 5' primer sop1-17 5'-ct tat gtt cca cgg tat cat caa tcc-3' and the 3' primer sop1-28 5'-c ttc tcc ttt act tcc tcc gta ctg att tgg tgg ttg ttg gtt g-3', for dpy-22::gfp. gfp fragments included the unc-54 3' untranslated region, and were amplified by PCR from pPD95.79 with the 5' primer sop1-34 5'-aat cag tac gga gga agt aaa gga gaa gaa ctt ttc act gg-3', for dpy-22::gfp, and the 3' primer unc54-5 5'-a taa gaa tgc ggc cgc aaa cag tta tgt ttg gta tat tgg gaa tg-3'. The individual fragments were purified by agarose gel electrophoresis, mixed in the appropriate combinations, and then subjected to PCR in the presence of the 5' primer sop1-17, and the 3' primer unc54-5. The dpy-22::gfp fusion was digested with NotI and cloned into NotI-digested pBRsop1FL. --precise ends. | ||
| Clone | pPD95.79 | ||
| F47A4 | |||
| Used_for | Transgene_construct | WBTransgene00027975 | |
| Reference | WBPaper00005632 |
