WormBase Tree Display for Construct: WBCnstr00010902
expand all nodes | collapse all nodes | view schema
| WBCnstr00010902 | Other_name | Expr2691_Ex | |
|---|---|---|---|
| Summary | [unc-59::gfp] | ||
| Driven_by_gene | WBGene00006793 | ||
| Gene | WBGene00006793 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [unc-59::gfp] translational fusion. To make an UNC-59::GFP translational fusion construct, a 5895-base pair (bp) region of genomic DNA containing the unc-59 open reading frame W09C5.2 plus 1094 bp upstream and 763 bp of downstream DNA was amplified from a single N2 worm by using PfuTurbo DNA polymerase. The upstream primer contained a SpeI restriction site, while the downstream primer had a PstI site, both used to clone the resulting fragment into the corresponding sites in pBluescript II SK+. Subsequently, a fragment containing the GFP sequence with artificial C. elegans introns was amplified from the vector pPD118.33 with PfuTurbo DNA polymerase using primers suitable for in frame insertion of the reaction product immediately before the stop codon of unc-59 to create plasmid pKK77. pKK77 was mutagenized to excise the unc-59 coding region, creating pKK83. --precise ends. | ||
| Clone | pPD118.33 | ||
| Used_for | Transgene_construct | WBTransgene00028129 | |
| Reference | WBPaper00006087 |
