WormBase Tree Display for Construct: WBCnstr00010990
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| WBCnstr00010990 | Other_name | Expr2859_Ex | |
|---|---|---|---|
| Summary | [unc-122::gfp] | ||
| Driven_by_gene | WBGene00006845 | ||
| Gene | WBGene00006845 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [unc-122::gfp] translational fusion. The unc-122::gfp reporter gene construct contains an 800 bp fragment upstream of the ATG start codon of unc-122 that was amplified by PCR(5 -gttttcccgctgataaattg-3 and 5 -gaagttcgtcaggagacca-3 ) and subcloned into the gfp vector pPD95.75. The translational GFP reporter containing the promoter and all of the genomic region up to the stop codon fused to the N terminus of GFP was constructed by subcloning the genomic PCR fragment (5 -gttttcccgctgataaattg-3 and 5 -tcaatatattctggtaagtcc-3 ) into the gfp vector pPD95.75. | ||
| Used_for | Transgene_construct | WBTransgene00028216 | |
| Transgene_coinjection | WBTransgene00026065 | ||
| WBTransgene00026070 | |||
| Reference | WBPaper00006427 |
