WormBase Tree Display for Construct: WBCnstr00011081
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| WBCnstr00011081 | Other_name | Expr3026_Ex | |
|---|---|---|---|
| Summary | [srf-3::gfp] | ||
| Driven_by_gene | WBGene00005153 | ||
| Gene | WBGene00005153 | ||
| Fusion_reporter | GFP | ||
| LacZ | |||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [srf-3::gfp] and [srf-3::LacZ] translational fusion constructs. To tag SRF-3 at the N terminus with GFP, 2.5-kb of srf-3 promoter sequence was PCR-amplified with primers that introduced a SalI site upstream and an EcoRV immediately downstream of the ATG. This PCR product was inserted into pPD118.15 (SalI- and Acc65I-blunted), creating the plasmid pBY1603. The SRF-3 coding region plus 1.7-kb of untranslated sequence were PCR-amplified and cloned via NheI and AatII into pBY1603, resulting in the plasmid pBY1605. To tag SRF-3 at the C terminus with {beta}-galactosidase carrying a nuclear localization signal, the srf-3 genomic region, including 2.5-kb of promoter sequence, was PCR-amplified. The primers were designed so that the stop codon was removed, and SalI sites were introduced at both ends of the PCR product. The PCR product was inserted into pPD95.57, creating pBY1907. | ||
| Clone | pPD95.57 | ||
| pPD118.15 | |||
| Used_for | Transgene_construct | WBTransgene00028307 | |
| Reference | WBPaper00024246 |
