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WormBase Tree Display for Construct: WBCnstr00012243

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Name Class

WBCnstr00012243Public_namepKS2.15
Other_nameExpr4785_Ex
SummaryPunc-46::GFP-ntx]
Driven_by_geneWBGene00006782
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summaryKS2.15 was constructed using the GFP and unc-54 terminator sequence contained in pPD95.85. The GFP sequence was amplified with primers containing PinAI sites on both sides. The PinAI sites were engineered so that they were in frame to the endogenous PinAI site within the first exon of the unc-46 gene and contained the ATG start codon from GFP. The amplified GFP and unc-54 terminator was cloned into the PinAI site in the full-length rescuing subclone for unc-46. A GFP product should be produced that is fused with 24 amino acids of UNC-46 protein at the N terminus.
Used_forTransgene_constructWBTransgene00012603
ReferenceWBPaper00030788