WormBase Tree Display for Construct: WBCnstr00012503
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| WBCnstr00012503 | Other_name | Expr716_Ex | |
|---|---|---|---|
| Summary | [sax-3::gfp] | ||
| Driven_by_gene | WBGene00004729 | ||
| Gene | WBGene00004729 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [sax-3::gfp] translational fusion. A translational sax-3::GFP fusion was constructed by cloning a blunted Apa1-Eag1 fragment from Apa1-Pst1 R06A1 rescuing subclone in frame into the Sma1 site of the GFP expression vector pPD95.75. This construct contained the 3.8 kb of sax-3 upstream sequence present in the rescuing subclone, the first 667 amino acids of the sax-3 open reading frame including the 5 Ig domains and the first FNIII domain as well as the first 6 introns. This transgene does not include all of sax-3, so it may not fully reflect transcriptional and posttranscriptional controls on sax-3 expression. --precise ends. | ||
| Clone | R06A1 | ||
| Used_for | Transgene_construct | WBTransgene00029708 | |
| Reference | WBPaper00002997 |
