WormBase Tree Display for Construct: WBCnstr00013852
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| WBCnstr00013852 | Other_name | Expr9252_Ex | |
|---|---|---|---|
| Summary | [act-1::GFP] | ||
| Driven_by_gene | WBGene00000063 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [act-1::GFP] transcriptional fusion. The act-1 (T04C12.6) promoter region (2929 bp) was PCR amplified from genomic DNA (forward primer, actgGTCGACgatttattagattaggaggaac; reverse primer, actgGGTACCatgtacctgaaaaattaaattg; inserted SalI and KpnI restriction enzymes sites used for cloning are indicated in uppercase). This fragment was inserted into the C. elegans GFP expression vector pPD95.75 before the GFP coding sequence to generate the plasmid pIP52. --precise ends. | ||
| Clone | pPD95.75 | ||
| Used_for | Transgene_construct | WBTransgene00031030 | |
| Reference | WBPaper00035977 |
