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WormBase Tree Display for Construct: WBCnstr00014819

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Name Class

WBCnstr00014819Summary[Punc-129::fbn-1::gfp]
Driven_by_geneWBGene00006852
GeneWBGene00022816
Fusion_reporterGFP
Construction_summaryfbn-1 was obtained by RT-PCR from wild-type cDNA, NotI sites were introduced at the C termini of the gene and used to insert GFP. The unc-129 promoter contains a 2644 bp fragment upstream of the unc-129 start codon that was amplified by PCR using the following primers (ODS276, 5-GGGGGGGCATGCGGAAACATGATATCGACGGAC-3, and ODS277, 5-GGGGGGGGATCCcttgcttgctcttccaattttcc-3) (Sieburth et al., 2005).
Used_forTransgene_constructWBTransgene00015224
ReferenceWBPaper00041192