WormBase Tree Display for Construct: WBCnstr00014819
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WBCnstr00014819 | Summary | [Punc-129::fbn-1::gfp] | |
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Driven_by_gene | WBGene00006852 | ||
Gene | WBGene00022816 | ||
Fusion_reporter | GFP | ||
Construction_summary | fbn-1 was obtained by RT-PCR from wild-type cDNA, NotI sites were introduced at the C termini of the gene and used to insert GFP. The unc-129 promoter contains a 2644 bp fragment upstream of the unc-129 start codon that was amplified by PCR using the following primers (ODS276, 5-GGGGGGGCATGCGGAAACATGATATCGACGGAC-3, and ODS277, 5-GGGGGGGGATCCcttgcttgctcttccaattttcc-3) (Sieburth et al., 2005). | ||
Used_for | Transgene_construct | WBTransgene00015224 | |
Reference | WBPaper00041192 |