WormBase Tree Display for Construct: WBCnstr00015444
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| WBCnstr00015444 | Summary | [FRM394(Punc-17::NYFP::UNC-17(+)); FRM401(Psup-1::SUP-1(+)::CCFP; pBX; pBS] | |
|---|---|---|---|
| Driven_by_gene | WBGene00012759 | ||
| WBGene00006756 | |||
| Gene | WBGene00012759 | ||
| WBGene00006756 | |||
| Fusion_reporter | CFP | ||
| YFP | |||
| Construction_summary | The pET11a-link-NGFP and pMRBAD-link-CGFP clones were generously provided by the Regan lab (Magliery et al. 2005). The N-terminal portion of GFP (NGFP) was amplified from pET11a-link-NGFP, and cloned into the Fire expression vector pPD95.77. The unc-17 coding sequence from cDNA RM#51p was cloned downstream of the NGFP sequence to yield plasmid RM#935p. The NGFP was converted to NYFP using a fragment amplified from a YFP-containing clone as the primer in a QuikChange reaction. The C-terminal portion of GFP (CGFP) was amplified from pMRBAD-link-CGFP, and cloned into the Fire expression vector pPD95.77. The sup-1 coding sequence from yk1353e09 (without the termination codon) was cloned upstream of the CGFP sequence, with an 8-amino acid linker between SUP-1 and CGFP (RM#934p). The CGFP was then converted to CCFP using QuikChange. | ||
| Used_for | Transgene_construct | WBTransgene00015870 | |
| Reference | WBPaper00041593 |
