WormBase Tree Display for Construct: WBCnstr00015448
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WBCnstr00015448 | Summary | [FRM394(Punc-17::NYFP::UNC-17(+); pBX; pBS] | |
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Driven_by_gene | WBGene00006756 | ||
Gene | WBGene00006756 | ||
Fusion_reporter | YFP | ||
Construction_summary | The pET11a-link-NGFP clone was generously provided by the Regan lab (Magliery et al. 2005). The N-terminal portion of GFP (NGFP) was amplified from pET11a-link-NGFP, and cloned into the Fire expression vector pPD95.77. The unc-17 coding sequence from cDNA RM#51p was cloned downstream of the NGFP sequence to yield plasmid RM#935p. The NGFP was converted to NYFP using a fragment amplified from a YFP-containing clone as the primer in a QuikChange reaction. | ||
Used_for | Transgene_construct | WBTransgene00015874 | |
Reference | WBPaper00041593 |