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WormBase Tree Display for Construct: WBCnstr00015448

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Name Class

WBCnstr00015448Summary[FRM394(Punc-17::NYFP::UNC-17(+); pBX; pBS]
Driven_by_geneWBGene00006756
GeneWBGene00006756
Fusion_reporterYFP
Construction_summaryThe pET11a-link-NGFP clone was generously provided by the Regan lab (Magliery et al. 2005). The N-terminal portion of GFP (NGFP) was amplified from pET11a-link-NGFP, and cloned into the Fire expression vector pPD95.77. The unc-17 coding sequence from cDNA RM#51p was cloned downstream of the NGFP sequence to yield plasmid RM#935p. The NGFP was converted to NYFP using a fragment amplified from a YFP-containing clone as the primer in a QuikChange reaction.
Used_forTransgene_constructWBTransgene00015874
ReferenceWBPaper00041593