WormBase Tree Display for Construct: WBCnstr00017154
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WBCnstr00017154 | Summary | [Punc-17::smg-5] | |
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Driven_by_gene | WBGene00006756 | ||
Gene | WBGene00004883 | ||
UTR_3 | WBGene00004883 | ||
Construction_summary | Insertion was created by recombination of pCL121 at Mos1 allele site cxTi10882. A 1971-bp region of smg-5 genomic sequence and 3'-UTR was amplified from genomic DNA, using primers smg-5-AvrII-F and smg-5-3'utr-KpnI-R, and inserted into the AvrII/KpnI sites of pCFJ178 (Addgene) to generate pCL119. 3249 bp of unc-17 promoter sequence was then amplified using primers unc-17-SbfI-F and unc-17-pro-AvrII-R and inserted into the SbfI/AvrII sites of pCL119 to generate pCL121. | ||
Used_for | Transgene_construct | WBTransgene00017762 | |
Reference | WBPaper00042120 |