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WormBase Tree Display for Construct: WBCnstr00017154

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Name Class

WBCnstr00017154Summary[Punc-17::smg-5]
Driven_by_geneWBGene00006756
GeneWBGene00004883
UTR_3WBGene00004883
Construction_summaryInsertion was created by recombination of pCL121 at Mos1 allele site cxTi10882. A 1971-bp region of smg-5 genomic sequence and 3'-UTR was amplified from genomic DNA, using primers smg-5-AvrII-F and smg-5-3'utr-KpnI-R, and inserted into the AvrII/KpnI sites of pCFJ178 (Addgene) to generate pCL119. 3249 bp of unc-17 promoter sequence was then amplified using primers unc-17-SbfI-F and unc-17-pro-AvrII-R and inserted into the SbfI/AvrII sites of pCL119 to generate pCL121.
Used_forTransgene_constructWBTransgene00017762
ReferenceWBPaper00042120