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WormBase Tree Display for Construct: WBCnstr00017156

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Name Class

WBCnstr00017156Summary[Punc-4::smg-5]
Driven_by_geneWBGene00006744
GeneWBGene00004883
Construction_summaryInsertion was created by recombination of pCL147 at Mos1 allele site cxTi10882. A 1971-bp region of smg-5 genomic sequence and 3'-UTR was amplified from genomic DNA, using primers smg-5-AvrII-F and smg-5-3'utr-KpnI-R, and inserted into the AvrII/KpnI sites of pCFJ178 (Addgene) to generate pCL119. 2804 bp of unc-4 promoter sequence was amplified using the primers unc-4-pro-SbfI-F and unc-4-pro-AvrII-R and inserted into the SbfI/AvrII sites of pCL119 to generate pCL147.
Used_forTransgene_constructWBTransgene00017764
ReferenceWBPaper00042120