WormBase Tree Display for Construct: WBCnstr00017159
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| WBCnstr00017159 | Summary | [Punc-4::unc-4::let-858] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006744 | ||
| Gene | WBGene00006744 | ||
| WBGene00002957 | |||
| Construction_summary | Insertion was created by recombination of pCL136 at Mos1 allele site ttTi5605. 4188 bp of let-858 sequence was amplified from pPD118.60 (L3808, Addgene), using primers SbfI-let-858F and XhoI-let-858R and inserted into SbfI/XhoI sites of pCFJ151 to generate pCL123. Promoter and coding sequences were then inserted into the AvrII/SbfI sites of pCL123 to generate tagged transgenes. For unc-4, a 5205-bp region including 2807 bp of promoter and the entire unc-4 coding region was amplified from genomic DNA, using primers unc-4 pro-AvrII-F and unc-4 SbfI-R to generate pCL145. | ||
| Used_for | Transgene_construct | WBTransgene00017767 | |
| Reference | WBPaper00042120 |
