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WormBase Tree Display for Construct: WBCnstr00018014

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Name Class

WBCnstr00018014Summary[Pdat-1::SAX-3::GFP; myo-2::mcherry]
Driven_by_geneWBGene00000934
GeneWBGene00004729
Fusion_reporterGFP
Construction_summaryA 802 bp dat-1 promoter was amplified by KOD-PLUS Neo DNA polymerase (YOBOBO) from the genomic DNA of wildtype N2 worms. The dat-1 promoter was then inserted into the BamHI and NotI sites of pPD117.10 to generate pPD117.01-Pdat-1::GFP. The full coding sequence of sax-3 was amplified from wildtype cDNA, and was then subcloned into the NotI and AgeI sites of pPD117.01-Pdat-1::GFP to generate pPD117.01-Pdat-1::SAX-3::GFP.
Used_forTransgene_constructWBTransgene00018649
ReferenceWBPaper00042510