WormBase Tree Display for Construct: WBCnstr00018014
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WBCnstr00018014 | Summary | [Pdat-1::SAX-3::GFP; myo-2::mcherry] | |
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Driven_by_gene | WBGene00000934 | ||
Gene | WBGene00004729 | ||
Fusion_reporter | GFP | ||
Construction_summary | A 802 bp dat-1 promoter was amplified by KOD-PLUS Neo DNA polymerase (YOBOBO) from the genomic DNA of wildtype N2 worms. The dat-1 promoter was then inserted into the BamHI and NotI sites of pPD117.10 to generate pPD117.01-Pdat-1::GFP. The full coding sequence of sax-3 was amplified from wildtype cDNA, and was then subcloned into the NotI and AgeI sites of pPD117.01-Pdat-1::GFP to generate pPD117.01-Pdat-1::SAX-3::GFP. | ||
Used_for | Transgene_construct | WBTransgene00018649 | |
Reference | WBPaper00042510 |