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WormBase Tree Display for Construct: WBCnstr00018673

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Name Class

WBCnstr00018673Summary[GFP::vab-3 fosmid; elt-2::DsRed]
GeneWBGene00006870
Fusion_reporterGFP
Construction_summaryThe vab-3 fosmid reporter was generated by fosmid recombineering based in fosmid WRM0623aF12 (Tursun et al., 2009) using a gfp reporter fused to the N terminus of the protein and replacing the stop codon. It was injected at a concentration of 15 ng/ul, in combination with 2 ng/ml of elt-2::DsRed and 100 ng/ul OP50 gDNA (as filling DNA) to generate 3 extrachromosmal arrays (otEx5057, otEx5058, otEx5059) none of which rescue the vab-3(ot569) phenotype.
Used_forTransgene_constructWBTransgene00019336
ReferenceWBPaper00044482