WormBase Tree Display for Construct: WBCnstr00018673
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WBCnstr00018673 | Summary | [GFP::vab-3 fosmid; elt-2::DsRed] | |
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Gene | WBGene00006870 | ||
Fusion_reporter | GFP | ||
Construction_summary | The vab-3 fosmid reporter was generated by fosmid recombineering based in fosmid WRM0623aF12 (Tursun et al., 2009) using a gfp reporter fused to the N terminus of the protein and replacing the stop codon. It was injected at a concentration of 15 ng/ul, in combination with 2 ng/ml of elt-2::DsRed and 100 ng/ul OP50 gDNA (as filling DNA) to generate 3 extrachromosmal arrays (otEx5057, otEx5058, otEx5059) none of which rescue the vab-3(ot569) phenotype. | ||
Used_for | Transgene_construct | WBTransgene00019336 | |
Reference | WBPaper00044482 |