WormBase Tree Display for Construct: WBCnstr00019322
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| WBCnstr00019322 | Public_name | KG#686 | |
|---|---|---|---|
| Summary | [Punc-17::INS-22-Venus] | ||
| Driven_by_gene | WBGene00006756 | ||
| Gene | WBGene00002105 | ||
| Fusion_reporter | Venus | ||
| Construction_summary | Herculase II polymerase and primers engineered with restriction sites were used to amplify the 333 bp ins-22 gene (without its stop codon and with reading frame adjusted to allow fusion to downstream Venus) from N2 genomic DNA and cloned it into the Nhe I/Age I cut KG#424 unc-17::NLP-21-Venus (removing the 500 bp NLP-21 insert in the process and leaving the 7.7Kb unc-17::INS-22-Venus vector fragment. | ||
| Used_for | Transgene_construct | WBTransgene00021244 | |
| Reference | WBPaper00044466 |
